Journal: Disease Models & Mechanisms

Article Title: Alcoholic hepatitis and metabolic disturbance in female mice: a more tractable model than Nrf2 −/− animals

doi: 10.1242/dmm.046383

Figure Lengend Snippet: Exposure to LdC+EtOH diet induces a transient change in hepatocyte energy metabolism. Polar metabolites were extracted from frozen liver lobes from animals exposed to LdC diet alone at day 17 (LdC) or LdC+EtOH at days 13, 15 and 17. Data are averages of multiple samples prepared from single animals in the control groups and 3-4 animals on alcohol diets. All spectra were acquired at 300 K on a Bruker 600 MHz spectrometer with a TCI 1.7 mm z-PFG cryogenic probe using a cooled Bruker SampleJet autosampler. 1D 1 H NMR spectra were processed using the NMRlab and Metabolab programmes within Matlab, version R2016b (MathWorks, Massachusetts, USA). Resonances were assigned using Chenomx (Alberta, Canada, 2015). Assigned peaks were then integrated in all spectra and metabolite intensities were compared between samples obtained on days as indicated, from mice exposed to LdC alone or to LdC+EtOH. Data are mean±s.e.m. of the group and have been divided into indicated categories of metabolites. Effects of diet were compared by using two-way ANOVA, which confirmed that for all classes of metabolite there was a significant effect of diet on intensity of signal ( P <0.001 for all metabolites). Tukey's multiple comparison test was carried out to compare individual diets. Here, for all classes of metabolite control LdC was significantly different than day 13 and day 15 time points on LdC+EtOH. No significant differences were found for metabolites in LdC and LdC+EtOH at day 17. Exceptions were BCAA and AAA metabolisms, with P <0.001 LdC vs LdC+EtOH at day 17. AAA, aromatic amino acid; a.u., arbitrary units; BCAA, branched chain amino acid.

Article Snippet: 1D 1 H NMR spectra were processed using the NMRlab and Metabolab programmes within Matlab, version R2016b (MathWorks, Massachusetts, USA).

Techniques: Control, Comparison

Journal: Disease Models & Mechanisms

Article Title: Alcoholic hepatitis and metabolic disturbance in female mice: a more tractable model than Nrf2 −/− animals

doi: 10.1242/dmm.046383

Figure Lengend Snippet: Exposure to LdC+EtOH diet induces a transient change in hepatocyte energy metabolism. Polar metabolites were extracted from frozen liver lobes from animals exposed to LdC diet alone at day 17 (LdC) or LdC+EtOH at days 13, 15 and 17. Data are averages of multiple samples prepared from single animals in the control groups and 3-4 animals on alcohol diets. All spectra were acquired at 300 K on a Bruker 600 MHz spectrometer with a TCI 1.7 mm z-PFG cryogenic probe using a cooled Bruker SampleJet autosampler. 1D 1 H NMR spectra were processed using the NMRlab and Metabolab programmes within Matlab, version R2016b (MathWorks, Massachusetts, USA). Resonances were assigned using Chenomx (Alberta, Canada, 2015). Assigned peaks were then integrated in all spectra and metabolite intensities were compared between samples obtained on days as indicated, from mice exposed to LdC alone or to LdC+EtOH. Data are mean±s.e.m. of the group and have been divided into indicated categories of metabolites. Effects of diet were compared by using two-way ANOVA, which confirmed that for all classes of metabolite there was a significant effect of diet on intensity of signal ( P <0.001 for all metabolites). Tukey's multiple comparison test was carried out to compare individual diets. Here, for all classes of metabolite control LdC was significantly different than day 13 and day 15 time points on LdC+EtOH. No significant differences were found for metabolites in LdC and LdC+EtOH at day 17. Exceptions were BCAA and AAA metabolisms, with P <0.001 LdC vs LdC+EtOH at day 17. AAA, aromatic amino acid; a.u., arbitrary units; BCAA, branched chain amino acid.

Article Snippet: All spectra were acquired at 300 K on a Bruker 600 MHz spectrometer with a TCI 1.7 mm z-PFG cryogenic probe using a cooled Bruker SampleJet autosampler as previously described ( ).1D 1 H NMR spectra were processed using the NMRlab and Metabolab programmes within MATLAB (MathWorks, Massachusetts, USA).

Techniques:

Journal: Disease Models & Mechanisms

Article Title: Alcoholic hepatitis and metabolic disturbance in female mice: a more tractable model than Nrf2 −/− animals

doi: 10.1242/dmm.046383

Figure Lengend Snippet: Exposure to LdC+EtOH diet induces a transient change in hepatocyte energy metabolism. Polar metabolites were extracted from frozen liver lobes from animals exposed to LdC diet alone at day 17 (LdC) or LdC+EtOH at days 13, 15 and 17. Data are averages of multiple samples prepared from single animals in the control groups and 3-4 animals on alcohol diets. All spectra were acquired at 300 K on a Bruker 600 MHz spectrometer with a TCI 1.7 mm z-PFG cryogenic probe using a cooled Bruker SampleJet autosampler. 1D 1 H NMR spectra were processed using the NMRlab and Metabolab programmes within Matlab, version R2016b (MathWorks, Massachusetts, USA). Resonances were assigned using Chenomx (Alberta, Canada, 2015). Assigned peaks were then integrated in all spectra and metabolite intensities were compared between samples obtained on days as indicated, from mice exposed to LdC alone or to LdC+EtOH. Data are mean±s.e.m. of the group and have been divided into indicated categories of metabolites. Effects of diet were compared by using two-way ANOVA, which confirmed that for all classes of metabolite there was a significant effect of diet on intensity of signal ( P <0.001 for all metabolites). Tukey's multiple comparison test was carried out to compare individual diets. Here, for all classes of metabolite control LdC was significantly different than day 13 and day 15 time points on LdC+EtOH. No significant differences were found for metabolites in LdC and LdC+EtOH at day 17. Exceptions were BCAA and AAA metabolisms, with P <0.001 LdC vs LdC+EtOH at day 17. AAA, aromatic amino acid; a.u., arbitrary units; BCAA, branched chain amino acid.

Article Snippet: All spectra were acquired at 300 K on a Bruker 600 MHz spectrometer with a TCI 1.7 mm z-PFG cryogenic probe using a cooled Bruker SampleJet autosampler as previously described ( ).1D 1 H NMR spectra were processed using the NMRlab and Metabolab programmes within MATLAB (MathWorks, Massachusetts, USA).

Techniques: